NLDMseq

[PMID:26475308] [BMC Bioinformatics]

Improving RNA-Seq expression estimation by modeling isoform- and exon-specific read sequencing rate

It is a latent variable model, “adopts latent variables to model the unknown isoforms, from which reads originate, and the underlying percentage of multiple spliced variants. The isoform- and exon-specific read sequencing biases are modeled to account for the non-uniformity of read distribution, and are identified by utilizing the replicate information of multiple lanes of a single library run.” See also this group’s PLoS ONE paper this year [PMID:26448625].

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