RNA-seq Biological Replicates

[PMID:27022035] [RNA]

How many biological replicates are needed in an RNA-seq experiment and which differential expression tool should you use

“more than six biological replicates should be used, rising to more than 12 when it is important to identify SDE genes for all fold changes. If less than 12 replicates are used, a superior combination of true positive and false positive performances makes edgeR the leading tool. For higher replicate numbers, minimizing false positives is more important and DESeq marginally outperforms the other tools.”


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