LoF Genetic Tools

[PMID:27795562] [Nature Reviews Genetics]

Loss-of-function genetic tools for animal models: cross-species and cross-platform differences

“Although all LOF methods reduce gene activity, the choice of approach (for example, mutagenesis, CRISPR-based gene editing, RNA interference, morpholinos or pharmacological inhibition) can have a major effect on phenotypic outcomes. Interpretation of the LOF phenotype must take into account the biological process that is targeted by each method. The practicality and efficiency of LOF methods also vary considerably between model systems.”

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